TaVrt2, an SVP-like gene, cooperates with TaVrn1 to regulate vernalization-induced flowering in wheat
为进一步贯彻落实人才强所战略,凝聚和吸引一批有科研潜能的优秀博士毕业生来所从事博士后研究工作,鼓励和支持在站博士后不断取得高水平科研成果,充分发挥博士后人才储备库作用,作科所面向国内外常年招收博士后研究人员。取得突出科研产出的博士后,可申请作科所优秀博士后奖励资助,具体如下:
一、申报对象
拟进站博士生、所内在站博士后
二、申报条件
1.正式进站前,近三年内以第一作者或通讯作者发表过高水平学术论文,或取得突出科研产出。
2.在站期间以独立第作者:Li Xie, Yong Zhang, Ke Wang1, Xumei Luo, Dengan Xu, Xiuling Tian, Lingli Li, Xingguo Ye, Xianchun Xia, Wenxue Li, Liuling Yan and Shuanghe Cao*
影响因子:7.433
刊物名称:New Phytologist
出版年份:26 November 2019
doi: 10.1111/nph.16339
文章摘要: TaVrn1 , encoding a MADS-box transcription factor (TF), is the central regulator of wheat vernalization-induced flowering. Considering that the MADS-box TF usually works by forming hetero or homo dimers, we conducted yeast two hybrid screening and identified an SVP-like MADS-box protein TaVrt2 interacting with TaVrn1. However, the specific function of TaVrt2 and the biological implication of its interaction with TaVrn1 remained unknown. We validated the function of TaVrt2 and TaVrn1 by wheat transgenic experiments and their interaction through multiple protein-binding assays. Population genetic analysis was also used to display their interplay. Transcriptomic sequencing and chromatin immunoprecipitation assays were performed to identify their common targets. TaVrt2 and TaVrn1 are flowering promoters in the vernalization pathway and interact physically in vitro , in planta and in wheat cells. Additionally, TaVrt2 and TaVrn1 were significantly induced in leaves by vernalization, suggesting their spatio-temporal interaction during vernalization. Genetic analysis indicated that TaVrt2 and TaVrn1 had significant epistatic effects on flowering time. Furthermore, native TaVrn1 was up-regulated significantly in TaVrn1 -OE (overexpression) and TaVrt2 -OE lines. Moreover, TaVrt2 could bind with TaVrn1 promoter directly. A TaVrt2 -mediated positive feedback loop of TaVrn1 during vernalization was proposed, providing additional understanding on the regulatory mechanism underlying vernalization-reduced flowering.
一作者或独立通讯作者发表了以作科所为第一单位的高水平学术论文,或取得突出科研产出。
三、资助标准
年薪不低于25万元;特别优秀者可直接入编并晋升副研究员或研究员
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